【文獻(xiàn)速遞】【NBT】【2022年】【7-12月】
聲明：本專欄主要對(duì)生命科學(xué)領(lǐng)域的一些期刊文章標(biāo)題進(jìn)行翻譯，所有內(nèi)容均由本人手工整理翻譯。由于本人專業(yè)為生物分析相關(guān)，其他領(lǐng)域如果出現(xiàn)翻譯錯(cuò)誤請(qǐng)諒解。
具有持久力的熒光蛋白
Micrograph of Cytaeis uchidae. A fluorescent protein expressed by this hydrozoan forms the basis of StayGold, a fluorescent protein with substantially increased photostability engineered by Hirano et al.
Cytaeis uchidae的顯微照片。這種水生動(dòng)物表達(dá)的熒光蛋白構(gòu)成了StayGold的基礎(chǔ)，StayGold是一種熒光蛋白，由Hirano 等人設(shè)計(jì)，具有顯著提高的光穩(wěn)定性。
1.Smartphone apps in the COVID-19 pandemic.
COVID-19大流行中的智能手機(jī)應(yīng)用程序。
2.SignalP 6.0 predicts all five types of signal peptides using protein language models.
SignalP 6.0使用蛋白質(zhì)語(yǔ)言模型預(yù)測(cè)所有五種類型的信號(hào)肽。
3.Fast nanopore sequencing data analysis with SLOW5.
使用SLOW5進(jìn)行快速納米孔測(cè)序數(shù)據(jù)分析。
4.Mitochondrial variant enrichment from high-throughput single-cell RNA sequencing resolves clonal populations.
來(lái)自高通量單細(xì)胞RNA測(cè)序的線粒體變異富集解決了克隆群體。
5.Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing.
通過(guò)超快納米孔基因組測(cè)序加速鑒定引起疾病的變體。
6.Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells.
源自干細(xì)胞的人胰島的功能、代謝和轉(zhuǎn)錄成熟。
7.A comparison of experimental assays and analytical methods for genome-wide identification of active enhancers.
對(duì)活性增強(qiáng)子的全基因組鑒定的實(shí)驗(yàn)測(cè)定和分析方法的比較。
8.CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information.
COSPAR確定了單細(xì)胞轉(zhuǎn)錄組和譜系信息的早期細(xì)胞命運(yùn)偏差。
9.Multiplex de Bruijn graphs enable genome assembly from long, high-fidelity reads.
多重de Bruijn圖可從長(zhǎng)長(zhǎng)的高保真讀取中啟用基因組組件。
10.Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue.
單核同工型RNA測(cè)序解鎖冷凍腦組織中的條形碼外顯子連接。
11.Endogenous ADAR-mediated RNA editing in non-human primates using stereopure chemically modified oligonucleotides.
內(nèi)源性ADAR介導(dǎo)的RNA編輯在非人類靈長(zhǎng)類動(dòng)物中使用立體純化學(xué)修飾的寡核苷酸。。
12.Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells.
合成內(nèi)含子可以實(shí)現(xiàn)剪接因子突變依賴性癌細(xì)胞的靶向。
13.Learning protein fitness models from evolutionary and assay-labeled data.
從進(jìn)化和測(cè)定標(biāo)記的數(shù)據(jù)中學(xué)習(xí)蛋白質(zhì)適應(yīng)度模型。
14.Designing sensitive viral diagnostics with machine learning.
通過(guò)機(jī)器學(xué)習(xí)設(shè)計(jì)敏感的病毒診斷。
15.A highly photostable and bright green fluorescent protein.
高度光穩(wěn)定且明亮的綠色熒光蛋白。
16.Computationally designed dual-color MRI reporters for noninvasive imaging of transgene expression.
計(jì)算設(shè)計(jì)的雙色MRI報(bào)告子用于轉(zhuǎn)基因表達(dá)的非侵入性成像。
Artistic impression of the Deep Visual Proteomics method developed by Mund et al., which is based on a combination of imaging, deep learning and mass spectrometry. Single cells are segmented, cell-typed, excised from tissue and analyzed for their protein content.
Mund等人開發(fā)的深度視覺(jué)蛋白質(zhì)組學(xué)方法的藝術(shù)效果圖，該方法基于成像、深度學(xué)習(xí)和質(zhì)譜的結(jié)合。單個(gè)細(xì)胞被分割、分型、從組織中切除并分析其蛋白質(zhì)含量。
1.Spatial charting of single-cell transcriptomes in tissues.
組織中單細(xì)胞轉(zhuǎn)錄組的空間圖表。
2.Integrative spatial analysis of cell morphologies and transcriptional states with MUSE.
使用MUSE對(duì)細(xì)胞形態(tài)和轉(zhuǎn)錄狀態(tài)的整合空間分析。
3.m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome.
在單基堿分辨率下測(cè)量整個(gè)哺乳動(dòng)物轉(zhuǎn)錄組的M6A RNA修飾。
4.Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro.
用scCUT和Tag-pro表征染色質(zhì)狀態(tài)的細(xì)胞異質(zhì)性。
5.Deep Visual Proteomics defines single-cell identity and heterogeneity.
深視覺(jué)蛋白質(zhì)組學(xué)定義了單細(xì)胞的身份和異質(zhì)性。
6.In vivo engineered B cells secrete high titers of broadly neutralizing anti-HIV antibodies in mice.
體內(nèi)工程的B細(xì)胞在小鼠中分泌高滴定度的廣泛中和抗HIV抗體。
7.Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells.
用于快速在體內(nèi)生產(chǎn)和釋放CAR-T細(xì)胞的生物學(xué)結(jié)構(gòu)可植入的支架。
8.A programmable encapsulation system improves delivery of therapeutic bacteria in mice.
可編程封裝系統(tǒng)改善了治療性細(xì)菌在小鼠體內(nèi)的輸送。
9.Efficient discovery of SARS-CoV-2-neutralizing antibodies via B cell receptor sequencing and ligand blocking.
通過(guò)B細(xì)胞受體測(cè)序和配體阻斷有效發(fā)現(xiàn)SARS-COV-2中和抗體。
10.Accurate detection of tumor-specific gene fusions reveals strongly immunogenic personal neo-antigens.
腫瘤特異性基因融合的準(zhǔn)確檢測(cè)顯示出強(qiáng)烈的免疫原性的個(gè)人新抗原。
11.Fludarabine increases nuclease-free AAV- and CRISPR/Cas9-mediated homologous recombination in mice.
氟達(dá)拉濱增加了小鼠中無(wú)核酸酶AAV和CRISPR/CAS9介導(dǎo)的同源重組。
Mt. Everest’s East Rongbuk Glacier was one of the glaciers sampled to create the comprehensive Tibetan Glacier Genome and Gene (TG2G) catalog, an inventory of microbial genetic diversity in this region.
珠穆朗瑪峰的東絨布冰川是為創(chuàng)建全面的西藏冰川基因組和基因（TG2G）目錄而采樣的冰川之一，該目錄是該地區(qū)微生物遺傳多樣性的清單。
1.Haplotype-resolved assembly of diploid genomes without parental data.
沒(méi)有父母數(shù)據(jù)的二倍體基因組的單倍型解析組裝。
2.Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants.
熱力學(xué)耦合生物傳感器，用于檢測(cè)針對(duì)SARS-CoV-2變體的中和抗體。
3.A genome and gene catalog of glacier microbiomes.
冰川微生物組的基因組和基因目錄。
4.Spatially informed cell-type deconvolution for spatial transcriptomics.
空間轉(zhuǎn)錄組學(xué)的空間信息的細(xì)胞類型反卷積。
5.DestVI identifies continuums of cell types in spatial transcriptomics data.
DESTVI在空間轉(zhuǎn)錄組學(xué)數(shù)據(jù)中識(shí)別細(xì)胞類型的連續(xù)體。
6.Generation of a live attenuated influenza A vaccine by proteolysis targeting.
通過(guò)蛋白水解靶向產(chǎn)生活體流感A疫苗。
7.CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA.
無(wú)CRISPR基礎(chǔ)編輯者具有增強(qiáng)的活性并擴(kuò)大了線粒體和核DNA的靶向范圍。
8.A split prime editor with untethered reverse transcriptase and circular RNA template.
具有不受束縛的逆轉(zhuǎn)錄酶和圓形RNA模板的拆分主編輯器。
9.An engineered prime editor with enhanced editing efficiency in plants.
工程的引導(dǎo)編輯器具有增強(qiáng)的植物編輯效率。
10.Targeting a gene regulatory element enhances rice grain yield by decoupling panicle number and size.
靶向基因調(diào)節(jié)元件可通過(guò)解耦穗數(shù)和大小來(lái)增強(qiáng)水稻顆粒的產(chǎn)量。
RNA治療劑的肝外遞送
A model of 2′-O-hexadecyl (C16)-conjugated siRNA (orange) in complex with RISC (white) and mRNA (green). Brown et al. show that C16-conjugated siRNA achieves efficient, durable targeted gene silencing in the central nervous system, eye and lung.
與RISC（白色）和mRNA（綠色）復(fù)合的2′-O-十六烷基（C16）-偶聯(lián)siRNA（橙色）模型。Brown等人展示C16偶聯(lián)的siRNA在中樞神經(jīng)系統(tǒng)、眼睛和肺中實(shí)現(xiàn)了高效、持久的靶向基因沉默。
1.Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes.
評(píng)估雄性沃爾巴契亞感染蚊子的生產(chǎn)和釋放的自動(dòng)化過(guò)程的效率。
2.Reply to: Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes.
回復(fù)：評(píng)估雄性沃爾巴契亞感染蚊子的生產(chǎn)和釋放的自動(dòng)化過(guò)程的效率。
3.Fast and highly sensitive full-length single-cell RNA sequencing using.
使用FLASH-seq進(jìn)行快速且高度敏感的全長(zhǎng)單細(xì)胞RNA測(cè)序。
4.Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress.
用Smart-Seq3xpress的完整轉(zhuǎn)錄本的可擴(kuò)展單細(xì)胞RNA測(cè)序。
5.Multi-omics single-cell data integration and regulatory inference with graph-linked embedding.
多組學(xué)單細(xì)胞數(shù)據(jù)集成和圖鏈接嵌入的監(jiān)管推斷。
6.DIALOGUE maps multicellular programs in tissue from single-cell or spatial transcriptomics data.
DIALOGUE映射單細(xì)胞或空間轉(zhuǎn)錄組數(shù)據(jù)的組織中的多細(xì)胞程序。
7.Comparison and imputation-aided integration of five commercial platforms for targeted DNA methylome analysis.
用于靶向DNA甲基化組分析的五個(gè)商業(yè)平臺(tái)的比較和插補(bǔ)輔助整合。
8.Identifying synergistic high-order 3D chromatin conformations from genome-scale nanopore concatemer sequencing.
從基因組尺度納米多聯(lián)體測(cè)序中鑒定出協(xié)同的高階3D染色質(zhì)構(gòu)象。
9.Expanding RNAi therapeutics to extrahepatic tissues with lipophilic conjugates.
用親脂性結(jié)合物擴(kuò)展RNAi療法到肝外組織。
10.Logic-gated antibody pairs that selectively act on cells co-expressing two antigens.
邏輯門控抗體對(duì)有選擇地作用于共表達(dá)兩種抗原的細(xì)胞。
11.Prediction of protein–ligand binding affinity from sequencing data with interpretable machine learning.
通過(guò)可解釋的機(jī)器學(xué)習(xí)來(lái)測(cè)序數(shù)據(jù)的蛋白質(zhì) - 配體結(jié)合親和力的預(yù)測(cè)。
Artistic rendering of a system for protein structure prediction. Chowdhury et al. present a deep learning method to predict a protein’s structure from its sequence alone, with applications to orphan and de novo–designed proteins.
1.Unlocking the promise of mRNA therapeutics.
解鎖mRNA療法的承諾。
2.Directed evolution and selection of biostable L-DNA aptamers with a mirror-image DNA polymerase.
具有鏡像DNA聚合酶的可生物固定L-DNA適體的定向演化和選擇。
3.Transplantation of a human liver following 3 days of ex situ normothermic preservation.
在原位正常溫度保存3天后，人肝移植。
4.Single-sequence protein structure prediction using a language model and deep learning.
單序蛋白結(jié)構(gòu)使用語(yǔ)言模型和深度學(xué)習(xí)預(yù)測(cè)。
5.Estimation of tumor cell total mRNA expression in 15 cancer types predicts disease progression.
腫瘤細(xì)胞總mRNA表達(dá)15種癌癥類型的估計(jì)預(yù)測(cè)疾病進(jìn)展。
6.Genome-wide mapping of somatic mutation rates uncovers drivers of cancer.
體細(xì)胞突變率的全基因組映射發(fā)現(xiàn)了癌癥的驅(qū)動(dòng)因素。
7.Variant to function mapping at single-cell resolution through network propagation.
通過(guò)網(wǎng)絡(luò)傳播在單細(xì)胞分辨率下函數(shù)映射的變體。
8.Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes.
活細(xì)胞和組織的時(shí)空多重免疫熒光成像，具有熒光探針的生物正交循環(huán)。
9.Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping.
深層組織多光子成像使用具有直接焦點(diǎn)傳感和塑形的自適應(yīng)光學(xué)器件。
10.A red light–responsive photoswitch for deep tissue optogenetics.
用于深層組織光遺傳學(xué)的紅光響應(yīng)光電開關(guān)。
11.Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR.
全血PCR對(duì)SARS-COV-2細(xì)胞免疫的快速，可擴(kuò)展的評(píng)估。
Color-enhanced image showing single-cell fluorescence measurements of protein and mRNA in the human lymph node. He et al. resolve RNA and protein localization at the subcellular level using multiple cycles of nucleic acid hybridization of fluorescent molecular barcodes.
彩色增強(qiáng)圖像顯示人淋巴結(jié)中蛋白質(zhì)和mRNA的單細(xì)胞熒光測(cè)量。He等人使用熒光分子條形碼的多循環(huán)核酸雜交解決亞細(xì)胞水平的RNA和蛋白質(zhì)定位。
1.Enhancing untargeted metabolomics using metadata-based source annotation.
使用基于元數(shù)據(jù)的源注釋來(lái)增強(qiáng)非靶向代謝組學(xué)。
2.High-throughput total RNA sequencing in single cells using VASA-seq.
使用VASA-seq在單細(xì)胞中進(jìn)行高通量總RNA測(cè)序。
3.High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging.
通過(guò)空間分子成像在固定組織中的亞細(xì)胞分辨率下的RNA和蛋白質(zhì)的高復(fù)合成像。
a．SMI ISH探針和報(bào)告子的設(shè)計(jì)原理圖。ISH探針包含和靶標(biāo)結(jié)合以及讀取區(qū)域，DNA序列的前35-50堿基用于和靶標(biāo)RNA結(jié)合，后60-80堿基序列包含4個(gè)連續(xù)的10-20堿基的報(bào)告子結(jié)合域，每一個(gè)結(jié)合域可以和獨(dú)特的報(bào)告子雜交。使用64位的條碼設(shè)計(jì)，總共有64種獨(dú)特的報(bào)告子結(jié)合域。每個(gè)報(bào)告基因都是一個(gè)單色分支結(jié)構(gòu)，寡核苷酸與四種熒光團(tuán)中的一種結(jié)合，在SMI圖像中將被檢測(cè)為四種顏色（藍(lán)色、綠色、黃色或紅色）中的一種。每個(gè)報(bào)告基因都有數(shù)量可控的15-60種染料和六個(gè)PC位點(diǎn)，可通過(guò)紫外線照射和每次循環(huán)報(bào)告基因讀數(shù)前的洗滌步驟有效淬滅信號(hào)。b. SMI RNA分析的工作流程表示。FFPE載玻片經(jīng)過(guò)標(biāo)準(zhǔn)組織制備，以暴露用于ISH探針雜交的RNA靶標(biāo)。樣品被組裝到流動(dòng)池中并加載到SMI儀器上，用于與16組報(bào)告基因一起循環(huán)讀出。因?yàn)槊總€(gè)報(bào)告基因組包含四個(gè)具有四種不同熒光團(tuán)的報(bào)告基因，所以在SMI測(cè)定中使用了64個(gè)獨(dú)特的報(bào)告基因來(lái)結(jié)合ISH探針上不同的報(bào)告基因結(jié)合域。在每組報(bào)告子雜交后，獲取高分辨率Z堆疊圖像，然后在與下一組報(bào)告子孵化之前切割和去除報(bào)告子的熒光團(tuán)。
4.Frequent aneuploidy in primary human T cells after CRISPR–Cas9 cleavage.
CRISPR – CAS9裂解后原代人T細(xì)胞中頻繁的非整倍性。
5.Viral variant-resolved wastewater surveillance of SARS-CoV-2 at national scale.
在國(guó)家范圍內(nèi)對(duì)SARS-CoV-2進(jìn)行病毒變體解析的廢水監(jiān)測(cè)。
6.A multiplex implantable microdevice assay identifies synergistic combinations of cancer immunotherapies and conventional drugs.
多重植入式微裝置分析可以確定癌癥免疫療法和常規(guī)藥物的協(xié)同組合。
7.Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice.
RAS突變癌對(duì)小鼠V型ATP酶的小分子抑制敏感。
8.The trispecific DARPin ensovibep inhibits diverse SARS-CoV-2 variants.
三特異性DARPin恩索維普抑制了不同的SARS-CoV-2變體。
9.Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds.
植物激素受體作為可重新編程的支架用于快速生物傳感器開發(fā)。
10.Synthetic memory circuits for stable cell reprogramming in plants.
合成記憶電路，用于植物中穩(wěn)定的細(xì)胞重編程。
