English name:Actin Polymerization Biochem Kit

supplier:Cytoskeleton

Product No.:BK003

Specifications:KIT30-100assays

?

The Actin Polymerization Biochem Kit??is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be measured in a fluorimeter to follow polymerization over time. Also, by using preformed pyrene F-actin, it is possible to follow depolymerization. Both cell/tissue extracts and purified proteins can be added to the reaction mixture to identify their effect on actin polymerization. The components of the kit can also be used separately for other actin based assays such as a spin-down assays to detect F-actin binding proteins (see also BK001) or size exclusion chromatography to identify G-actin binding proteins. See the About Actin page for more information on assays testing actin binding proteins. While this kit comes with pyrene labeled skeletal muscle actin, it can also be used to study polymerization of other types of actin such as non-muscle actin (Cat. # APHL99) or cardiac actin (Cat. # AD99). Polymerization assays with these actins can be performed using a 10:1 ratio between the actin you want to study and the included pyrene actin

?

真核細胞的細胞骨架是與維持細胞形態(tài)結構和細胞運動有關的纖維網絡，由微絲、微管和中間纖維組成。肌動蛋白單體（G-Actin）一個接一個連成一串肌動蛋白鏈，而微絲是由兩股方向相同的肌動蛋白鏈以螺旋的形式組成的呈圓筒狀的纖維。微絲能被組裝和去組裝。當單體上結合的是ATP時，就會有較高的相互親和力，單體趨向于聚合成多聚體，就是組裝。而當ATP水解成ADP后，單體親和力就會下降，多聚體趨向解聚，即是去組裝。那么肌動蛋白是如何聚合與解聚的？

?

艾美捷Actin聚合檢測試劑盒的原理：

此肌動蛋白聚合分析生化試劑盒基于在聚合過程中pyrene（芘）結合Actin時熒光增強的原理。隨著時間的推移，在聚合過程中，當芘G-actin（單體）形成芘F-actin（纖維形肌動蛋白）時能夠檢測到熒光增強。此外，通過使用預先形成的芘F-actin，還可以進行解聚。肌動蛋白聚合分析生化試劑盒是一種非?？焖俸徒洕难芯考拥鞍拙酆?解聚的方案。

?

艾美捷Actin聚合檢測試劑盒的組分也可單獨用于其他基于肌動蛋白的測定，例如檢測F-肌動蛋白結合蛋白的旋轉下降測定（也見BK001）或用于鑒定G-肌動素結合蛋白的大小排阻色譜。

?

艾美捷Actin聚合檢測試劑盒化學性質：

中文名稱：Actin聚合生化盒試劑

英文名：Actin Polymerization Biochem Kit

cat#：BK003

規(guī)格：KIT30-100次檢測

保存建議：推薦常溫運輸，建議保存于4°C。

?

艾美捷Actin聚合檢測試劑盒的應用：

1.通過添加組織提取物，肌動蛋白結合蛋白或化合物來分析其對Actin聚合或解聚的影響。

2.通過添加F-actin成核蛋白，化合物或提取物來分析其對Actin聚合的影響。

3.通過添加F-肌動蛋白切斷蛋白，化合物或組織提取物來分析其對F-actin穩(wěn)態(tài)水平的影響。

4.確定在各種實驗條件下肌動蛋白聚合的臨界濃度。