秀麗隱桿線蟲蛋白抗體的應(yīng)用類型和結(jié)果圖鑒賞
自?1965?年起，科學(xué)家?Sydney Brenner?將線蟲引入分子生物學(xué)和發(fā)育生物學(xué)研究領(lǐng)域。1983?年完成線蟲從受精卵到成體的細(xì)胞譜系，是發(fā)育生物學(xué)史上具有里程碑性的工作，隨后線蟲在胚胎發(fā)育、性別決定、細(xì)胞凋亡、行為與神經(jīng)生物學(xué)等方面研究中得到廣泛應(yīng)用，也是是衰老和壽命研究中最重要的模式生物。 秀麗線蟲因其遺傳背景清楚、個(gè)體結(jié)構(gòu)簡單、生活史短、基因組測序完成等，在遺傳與發(fā)育生物學(xué)、行為與神經(jīng)生物學(xué)、衰老與壽命、人類遺傳性疾病、病原體與生物機(jī)體的相互作用、藥物篩選、動(dòng)物的應(yīng)急反應(yīng)、環(huán)境生物學(xué)和信號(hào)傳導(dǎo)等領(lǐng)域得到廣泛應(yīng)用。
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以下是艾美捷Covalab?秀麗隱桿線蟲蛋白抗體：
蛋白靶標(biāo)?宿主?應(yīng)用?貨號(hào)
Cathepsin L Protease (mature region)?兔?EM, WB?pab0213
Cathepsin L Protease (mature region)?兔?EM, WB?pab0213-P
Cathepsin L Protease (pro region)?兔?EM, WB?pab0212
DAF-3?兔?ChIP, IP, WB?pab50051
Heat Shock Protein 70 (C92F3A-5)?小鼠?ELISA, FC, IHC, IP, WB?mab60253
Heat Shock Protein 70 (N27F3-4)?小鼠?IHC-P, IP, WB?mab70449
JNK1?兔?IP, WB?pab50054
Lamin-1?兔?ELISA, WB?pab0750-P
Phospholipase C / PLC210?兔?ELISA, WB?pab0599
Phospholipase C Protein 3 / PLC-3?兔?ELISA, WB?pab0600
Phospholipase C Protein 4 / PLC-4?兔?ELISA, WB?pab0598
Serine Protease Inhibitor / Bli5?兔?ELISA, WB?pab0789-P
Silent Information Regulator 2.1 / Sir2.1?兔?IHC, IP, WB?pab50052
Tubby Protein Homolog / TUB-1?兔?IP, WB?pab50053
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秀麗隱桿線蟲蛋白抗體應(yīng)用類型：
ChIP：Chromatin IP?染色質(zhì)免疫沉淀
ELISA：Enzyme-linked immunosorbent assay?酶聯(lián)免疫吸附測定
EM：Electron microscopy?電子顯微鏡
FC：Flow cytometry?流式細(xì)胞術(shù)
IHC：Immunohistochemistry?免疫組化（IHC-P：IHC, paraffin section?石蠟切片的免疫組化）
IP：Immunoprecipitation?免疫沉淀
WB：Western blot?蛋白質(zhì)印跡
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秀麗隱桿線蟲蛋白抗體結(jié)果圖鑒賞：
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秀麗隱桿線蟲蛋白抗體引用文章鑒賞：
Popara M et al. Lesser protein degradation machinery correlates with higher BM86 tick vaccine efficacy in Rhipicephalus annulatus when compared to Rhipicephalus microplus. Vaccine, 2013 Aug 22.
Britton C. and Murray L. Cathepsin L protease (CPL-1) is essential for yolk processing during embryogenesis in Caenorhabditis elegans.Journal of Cell Science 2004; 117, 5133-5143.
